Fig. 7
From: Lipid droplet and peroxisome biogenesis occur at the same ER subdomains
Pex30/MCTP2 sites simultaneously associate with LDs and Peroxisomes/PPVs. a Images of COS7 cells transiently co-transfected with plasmids expressing YFP-MCTP2 (RHD), LiveDrop-mCherry, and CFP-SKL (a peroxisome marker). Stacks of 20 images with a step size of 0.3 μm were deconvolved; images from single plane are shown. Bar = 5 μm. Graph shows percent association of CFP-SKL puncta associated with YFP-MCTP2(RHD) and LiveDrop-mCherry, from raw images or where the CFP-SKL images was rotated 90 degrees, n = 300 CFP-SKL punctae. b Images of WT and pex3atg1Δ cells expressing endogenously tagged Pex30-2xmCherry and Pex14-YFP (a PPV marker) and Erg6-BFP from plasmids. Stacks of three images with a step size of 0.25 μm were deconvolved; images from single plane are shown. Bar = 3 μm. c Quantification of experiments in b indicating percent association of Pex14-YFP punctae with Pex30-2xmCherry and Erg6-BFP, n = 100 cells
