Fig. 7
From: Insulin/Snail1 axis ameliorates fatty liver disease by epigenetically suppressing lipogenesis
Hepatic Snail1 ameliorates insulin resistance in obesity. a–d Snail1∆hep (n = 11) and Snail1flox/flox (n = 11) male mice (8–9 weeks) were fed a HFD for 10 weeks. a Overnight fasting plasma insulin levels. b ITT. AUC areas under curves. c GTT. d Mice were fasted overnight (14 h) and injected with insulin (0.5 units/kg). Liver extracts were prepared 5 min later and immunoblotted with anti-phospho-Akt (pSer473 or pThr308) or anti-Akt antibodies. Akt phosphorylation was normalized to total Akt levels. e, f C57BL/6 male mice (8–9 weeks) were fed a HFD for 7 weeks and transduced with Snail1 (n = 10) or GFP (n = 10) adenoviral vectors for 2 weeks after transduction. e ITT. f GTT. Data are presented as mean ± SEM. *p < 0.05, two-tailed unpaired Student’s t test
