Fig. 2
From: Myeloid apolipoprotein E controls dendritic cell antigen presentation and T cell activation
Hematopoietic deficiency of apoE promotes allograft rejection and allogenic T-cell expansion. a Graphic representation of skin allograft transplantation experiment: a piece of tail skin from C57BL/6Kd donor was transplanted on the back of bone marrow transplanted (BMT) WT and apoE KO (BMT) mice; graft survival was scored up to 3 weeks. b Percentage of graft survival following skin allotransplantation in BMT mice. Survival of <50% of the donor skin was recorded as rejection. c Plasma cholesterol determination (mg/dL) after allograft rejection in BMT mice. d FPLC profiles of cholesterol distribution in the different lipoprotein subclasses (VLDL, LDL and HDL) from pooled plasma of grafted BMT mice (n = 4 for each group). e and g Proliferation of CD4+ (e) and CD8+ (g) lymphocytes isolated from draining lymph nodes of BMT-grafted mice, determined as Ki67+ staining following challenge with splenocytes isolated from C57BL/6Kd donor. f and h Polarization of CD4+ (f) and CD8+ (h) lymphocytes isolated from draining lymph nodes of BMT-grafted mice toward TEM subset after challenge with splenocytes isolated from C57BL/6Kd donor. N = 4 per group. Statistical analysis was performed with Gehan-Breslow-Wilcoxon test (b) and two-way Anova (c, e–h). Data are reported as mean ± SEM; *p < 0.05, **p < 0.01
