Fig. 4

ApoE deficiency alters the phenotype of spleen-derived DCs. a Number of CD11c⁺ from WT and apoE KO spleen normalized by spleen weight. b Gating strategy for DC subsets phenotyping. c Number of cDC1 (CD11c⁺MHCII⁺B220^-CD11b⁻CD8⁺) and cDC2 (CD11c⁺MHCII⁺B220⁻CD11b⁺CD8⁻) corrected for spleen weight of WT or apoE KO mice. d Median fluorescence intensity (MFI) of MHCII in cDC1s and cDC2s from WT or apoE KO mice; representative histograms are shown. e Median fluorescence intensity (MFI) of MHCI in cDC1s and cDC2s from WT or apoE KO mice; representative histograms are shown. f Percentage of double positive GFP⁺/Eα:I MHCII⁺ BMDCs after in vitro antigen uptake and presentation assay with Eα peptide; representative contour plots are shown. g-h Median fluorescence intensity (MFI) of GFP (h) and MHCII (i) in DCs after in vivo antigen uptake and presentation assay with Eα peptide; representative contour plots and histograms are presented. N = 3 (f), N = 4 (g–h), N = 5 (a–e) per group. Statistical analysis was performed with unpaired T-test. Data are reported as mean ± SEM; *p < 0.05