Fig. 8
The absence of clathrin at endosomes results in aberrant ILV formation. a Budding profiles as observed in electron micrographs were binned into three morphological categories: Pits, U-shaped buds and omega-shaped buds (see also Supplementary Fig. 8). The outlines of these budding profiles were aligned with each other to assess their uniformity. Upper row: budding profiles from the control dataset. Lower row: budding profiles from the siHRS/HRS770 dataset. In all, 9–10 profiles per category and condition were used for superimposition. Neck diameter and depth were measured on all displayed profiles and their average ± SD is indicated. b Tomograms showing examples of every budding profile category. Scale bar, 20 nm. c Neck width of Omega-shaped buds represented as dot plot. Mean ± SD. t-test, n.s. not statistically significant. d Neck length of Omega-shaped buds represented as dot plot. Mean ± SD. t-test, *p < 0.05. e Pit width represented as dot plot. Mean ± SD. t-test, *p < 0.05. f Depth of U-shaped budding profiles represented as dot plot. Mean ± SD. t-test, ***p < 0.001
