Fig. 2

CA injection accelerated development of bone metastasis in a hind limb. a Representative BL images (left) and BL intensity in region of interest (ROI) (right) at indicated days after LLC/luc cells injection by CA (n = 16) or IC injection (n = 12). Red circles in the images of 0.5 h indicate ROI. *P < 0.05 (two-side student’s t-test). Error bars indicate s.e.m. b Metastatic lesions in hind limb at 7 days after CA or IC injection of LLC/luc cells. The average number of a metastatic lesion per bone section was shown above the bar graph. n = 23 (CA), n = 28 (IC), *P < 0.05 (two-side student’s t-test). c Representative fluorescence images (left) and quantitative analysis of bone metastasis in femur bones at 14 days after injecting LLC/mKO2-Rluc8.6 cells via CA or IC. Metastasis and bone were visualized with mKO2 fluorescence (red) and tissue autofluorescence (cyan), respectively. A scale bar is 500 μm. n = 8 (CA), n = 6 (IC). *P < 0.05 (two-side student’s t-test). d X-ray micro CT imaging (left) and quantitative analysis (right) of bone volume in the femur at 14 days after CA or IC injection of LLC/mKO2-Rluc8.6 cells. Representative images are transverse plane of femurs shown in c. n = 8 (CA), n = 6 (IC). *P < 0.05 (two-side student’s t-test). e Representative BL images of mice at 25 and 32 days after CA or IC injection of LLC/luc cells. The representative image of mice at 32 days after IC injection are not shown because all IC-injected mice died before the day. f Representative ex vivo BL images of organs harvested from a mouse at 32 days after CA injection of LLC/luc cells. A scale bar is 10 mm. V vesicular gland, B brain, Lg lung, T testis, K kidney, S spleen, Lv liver, SI stomach and intestine