Fig. 1

Mass spectrometry identified AGAP004551 in association with sporozoites purified from mosquito saliva. a An image depicting the collection of Plasmodium sporozoites from the saliva of infected mosquitoes in a pipet tip (sporozoites in red, scale 10 μm). b The mosquito proteins associated with sporozoites collected from saliva of infected mosquitoes (SPZ) were compared to residual mosquito proteins from naive saliva (naive) by LC MS-MS. Three independent biological replicates were used. Each Venn diagram represents the A. gambiae proteins associated with each fraction (n = 35–50 mosquitoes/sample). The numbers in red indicate proteins that were only present in the samples from Plasmodium-infected mosquitoes. c The A. gambiae proteins unique to each SPZ sample were then compared to each other to determine potential common proteins. d AGAP004551 was identified in all three experiments and several peptides were discerned in two of the experiments. The proteins identified in two of the independent experiments are highlighted in blue and green, respectively