Fig. 1
From: Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis
Hepatic Blnc1 is linked to obesity and lipogenic activation. a qPCR analysis of Blnc1 and Srebp1c expression in the livers from lean (open) and obese (filled) mice. For diet-induced obesity (DIO), WT mice were fed chow (n = 4) or HFD (n = 12) for 10 weeks. For genetic obesity, WT (n = 5), ob/ob (n = 3), or db/db (n = 6) mice were fed standard chow. Data represent mean ± SEM. **p < 0.01, ***p < 0.001, obese vs. lean, two-tailed unpaired Student’s t-test. b Correlation between liver Blnc1 RNA levels and body weight (left) or liver fat content (right) in HFD-fed mice. c qPCR analysis of gene expression in hepatocytes transduced with GFP (open) or Blnc1 (filled) adenovirus followed by treatment with vehicle (Veh) or 5 μM T0901317 (T1317) for 24 h. d Incorporation of 14C-acetate into lipids in transduced hepatocytes treated with Veh or T1317 for 24 h. Data in (c) and (d) represent mean ± SD (n = 3). **p < 0.01, ***p < 0.001, GFP vs. Blnc1, two-way ANOVA. e Plasma and liver TAG and cholesterol in mice transduced with AAV vectors expressing GFP (n = 5) or Blnc1 (n = 7). f Immunoblots of total liver lysates and liver nuclear extracts (NE) from transduced mice. g qPCR analysis of hepatic gene expression in transduced mice. Data in (e) and (g) represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, GFP vs. Blnc1, two-tailed unpaired Student’s t-test
