Fig. 2
From: Long noncoding RNA licensing of obesity-linked hepatic lipogenesis and NAFLD pathogenesis
Blnc1 is required for LXR-mediated lipogenic activation. a Plasma and liver TAG content from WT or Blnc1 KO mice receiving oral gavage of vehicle (Veh, open, WT, n = 4; KO, n = 3) or T0901317 (T1317, filled, WT, n = 3; KO, n = 4) for 4 days. b H&E staining of liver sections. Scale bar = 100 μm. c qPCR analysis of hepatic gene expression. Data in (a) and (c) represent mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001, WT vs. KO, two-way ANOVA. d Immunoblots of total liver lysates and liver nuclear extracts. e qPCR analysis of gene expression in WT and Blnc1 KO hepatocytes treated with vehicle (Veh) or 5 μM T1317 for 24 h. f Incorporation of 14C-acetate into lipids in hepatocytes treated with Veh or T1317. Data in (e, f) represent mean ± SD (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, WT vs. KO, two-way ANOVA. g qPCR analysis of gene expression in hepatocytes transduced with GFP or Blnc1 adenovirus treated with Veh or 10 μM SR9238 for 24 h. h Incorporation of 14C-acetate into lipids in treated hepatocytes. Data in (g) and (h) represent mean ± SD (n = 3). **p < 0.01, ***p < 0.001, GFP vs. Blnc1, #p < 0.001, Veh vs. SR9238, two-way ANOVA
