Fig. 7
From: Size control in mammalian cells involves modulation of both growth rate and cell cycle duration
Contribution of growth and time modulation in overall size control. a Replicative growth, log(Vmitosis/Vbirth) vs. logarithm of volume at birth log(Vbirth) for HT29-wt cells. The slope coefficient of the linear regression gives −λ and indicates the strength of the effective size control (−λ = −0.5 ± 0.002, R2 = 0.85, n = 132, N = 3). b Cell cycle duration τ vs. initial volume log(Vbirth) for HT29-wt cells. The slope coefficient of the linear regression gives −〈τ〉〈θ〉, with 〈τ〉 the average cell cycle duration and θ the strength of control by time modulation. A positive value of θ corresponds to a positive effect on size control (〈−τ〉θ = −7 ± 0.2, R2 = 0.88, n = 163, N = 3. c Growth rate α vs. volume at birth log(Vbirth), for a dataset on bacteria from ref.51. The slope coefficient of the linear regression gives −〈α〉〈γ〉, with 〈α〉 the average growth rate and γ the control due to growth rate modulations. A positive value of γ corresponds to a positive effect on size control (−〈α〉γ = −0.0005 ± 0.0002, R2 = 0.06, n = 2107). d Left: plot of θ 〈τ〉 〈α〉, vs. γ 〈τ〉 〈α〉 for the bacteria dataset shown in Fig. 7c. Positive values along both y and x axes correspond to a positive effect on size control via time or growth modulation respectively. Right: plot of θ multiplied by 〈G〉, the average replicative growth 〈G〉=〈log(Vmitosis)/log(Vbirth)〉, vs. γ multiplied by 〈G〉 for HT29-wt cells shown in a and b. e Comparison of datasets for bacteria (data from refs.8, 51,49,52) and yeasts (data from refs.11, 16), plotted as in d. Each point corresponds to a different growth condition (see Supplementary Fig. 8d). f Comparison of datasets for animal cells (our results and data from ref.30.), plotted as in Fig. 8d. a, b, c Dots are single-cell measurements, squares with error bars are median bins with s.d., and black lines show the linear regression performed on the median bins weighted by the number of observations in each bin. d–f The dashed lines indicate the threshold above which time modulation (horizontal line) and growth modulation (vertical line) have a positive effect on size control. Values are given as slope ± standard error. See also Supplementary Figure 8
