Fig. 6
Engineered T cell distribution and cytokine release in vivo. OVCAR-3 tumor-bearing mice were treated with 6.0 × 106 anti-HER2 28ζ CAR- or HER2-TAC-T cells, or a matched total number of vector control cells. Mice are sacrificed at 1, 3, 5, and 7 days post-ACT1 for multiplex serum cytokine analysis or perfusion and fixation of tissues for subsequent histology. a Multicolor IHC is performed on tumor and lung tissue 7 days post-ACT1. Tissues are stained for CD8 (cyan), CD4 (yellow), DNA (blue) and a proliferation marker (Ki-67, purple). Data are representative of 3 mice. Scale bar indicates 100 μm. b Multiplex analysis of human cytokines in mouse serum on day 3 and 7. Measurements that fall below 0.2 pg/mL are below the calibration range and are therefore defined as 0. 0 values are depicted on the graph’s x axis. Statistical analysis is provided in Supplementary Table 1, analysis was performed using multiple t-test. Individual data points are shown, bars indicate standard deviation and center bars indicate the median
