Fig. 7
DO-SRS microscopy visualizes in vivo protein and lipid metabolism simultaneously in C. elegans. a Cartoons depict germline development66 with the following color scheme: yellow mitotic region, light green transition (early prophase of meiosis I), dark green pachytene, dark blue spermatogenesis, and pink oogenesis. In adults, a color gradient from orange to dark yellow indicates the development (arrow) from newly fertilized eggs to 32-cell embryo, which would be expelled via the vulva (triangles). b L4 animals and day 0 adults were grown on 20% D2O plates for 3 h before imaging (dashed outline indicates the gonad). Scale bar = 20 μm. c Day 0–7 adults were grown on 20% D2O plates for 3 h and then imaged. Mean intensity ratios (mean ± s.d.) are shown for animals divided into four age groups. N = 10 in each group. d Day 0–8 adults were transferred from regular NGM plates to 20% D2O plates and then imaged in 3 h. The percentages of animals that showed CD signals in clumps in the body cavity and outside of the oocytes are shown; N = 8 for each time point. e SRS microscopic images of day 5 and day 8 adults after 3 h D2O probing. Arrows indicate newly formed lipid and protein accumulations only labeled by CD signals, whereas arrowheads indicate pre-existing mass labeled by CH signals. The color scale bars represent the scale for CDL/CHL and CDP/CHP ratios
