Table 3 Phenotype expression specification generated from gene arrays from FRCs
From: Exploring the role of stromal osmoregulation in cancer and disease using executable modelling
Cellular property | Expected change | Reasoning | Model expected results |
|---|---|---|---|
Viability | |||
ETDLN | Increase | Experiments show increasing number of cells initially (Riedel et al.35). Gene array results also suggest proliferation pathways are upregulated | Viability increase from 2 to 3 or 4 in the presence of early stage TFs |
LTDLN | No change/Increase | Experiments show cell number increase plateus at later stage. Gene array results show proliferation pathways different to ETDLN. (Riedel et al.35) | Viability value of 2, or increase to 3 or 4 for late stage TFs |
LPS | Increase | Proliferation helps swell the LN under immune conditions due to increasing number of FRCs. (Fletcher et al.39) | Viability increase from 2 to 3 or 4 in the presence of LPS |
Membrane dynamics/movement | |||
ETDLN | Increase | Deregulation of membrane remodelling pathways in the gene array. (Riedel et al.35) | Migration increase from 2 to 3 or 4 in the presence of early stage TFs |
LTDLN | Increase | Deregulation of membrane remodelling, as well as migration pathways in the gene array. (Riedel et al.35) | Migration increase from 2 to 3 or 4 in the presence of late stage TFs |
LPS | Increase | Deregulation of movement/migration pathways, including MMPs in the gene array. (Malhotra et al.36) | Migration increase from 2 to 3 or 4 in the presence of LPS |
Attachment | |||
ETDLN | Increase | Deregulation of junction molecules in gene array. (Riedel et al.35). Cell adhesive properties changed in attachment assay | Attachment increase from 2 to 3 or 4 in the presence of early stage TFs |
LTDLN | Increase | Deregulation of junction molecules in gene array. (Riedel et al.35). Cell adhesive properties changed in attachment assay | Attachment increase from 2 to 3 or 4 in the presence of late stage TFs |
LPS | Unknown | – | – |
Contractility | |||
ETDLN | No change | Contractility assay shows increase only in LTDLN, microarray shows collagen and associated contraction genes deregulated at LTDLN only (Riedel et al.35) | Contractility remains at value of 2, in the presence of early stage TFs |
LTDLN | Increase | Cell contractility assays show increase in ability to contract collagen gel. (Riedel et al.35) | Contractility increases from 2 to 3 or 4 in the presence of late stage TFs |
LPS | Decrease | Contractility shown to be decreased under immune activation on LN. (Fletcher et al.39, Astarita et al.38) | Contractility decreases from 2 to 1 or 0 in the presence of LPS |
Cell size | |||
ETDLN | No change | Light scattering experiments show no change in cell size. (Riedel et al.35) | Cell size value of 1 in the presence of early stage TFs |
LTDLN | No change | Light scattering experiments show no change in cell size. (Riedel et al.35) | Cell size value of 1 in the presence of late stage TFs |
LPS | Increase | Light scattering experiments show increase in cell size. (Acton et al.39) | Cell size value of 2 in the presence of LPS |
