Fig. 3 | Nature Communications

Fig. 3

From: Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Fig. 3

Cas9-mediated gene editing in A. aegypti by embryo injection and ReMOT Control. a Example phenotypes of individuals obtained after standard embryonic microinjection into A. aegypti embryos of Cas9, P2C-Cas9, and P2C-EGFP-Cas9 fusion RNP complexed with sgRNA460. From left to right: G0 white and wild-type larvae; mosaic pupae; wild-type and white pupae; mosaic adult; wild-type and white adults. b Genotypes of alleles obtained at the 460 site from selected recovered white-eye offspring. The allele 3Δ-10 was isolated in a colony of white-eye mosquitoes (Wh-iso8-kmo460). c Phenotypes of offspring obtained after female injection of ReMOT Control RNPs complexed with sgRNA519* into A. aegypti females. d Genotypes of obtained modified offspring. Note the maternal and paternal allele clone sequences of knockout individuals. Mosaic individuals showed both mutated sites and intact WT sequences in clones from the same mosquito

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