Fig. 5 | Nature Communications

Fig. 5

From: Targeted delivery of CRISPR-Cas9 ribonucleoprotein into arthropod ovaries for heritable germline gene editing

Fig. 5

Schematic description and model of ReMOT Control vs embryo injection for Cas9-mediated gene targeting. ReMOT Control-mediated and traditional gene-editing strategies are compared in terms of methods (injection stage and timing, equipment, reagents, screening). Generation designations (Gx) are indicated in brown letters at the top left of each panel. A schematic model of the uptake of P2C-Cas9 RNP into the mosquito oocyte is illustrated in the G0, ReMOT Control panel. PBF post-blood feeding, RNP ribonucleoprotein, sgRNA single guide RNA, HRMA high-resolution melting assay, HDR homology-directed repair

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