Fig. 7
From: JNK regulates muscle remodeling via myostatin/SMAD inhibition
SMAD2-linker phosphorylation with exercise humans. a Healthy young men underwent a standard session of endurance exercise (cycling; N = 8 per time point) or resistance exercise (weighted leg extension; N = 7 per time point). Skeletal muscle biopsies were taken before exercise (R; rest), immediately post-exercise (Time 0), and 15, 30, and 60 min post-exercise. b, c The time course of SMAD2-linker phosphorylation (pSMAD-L) (b) and JNK activation (pJNK) (c) was determined. Mean ± SEM are shown for (b) and (c). d Individual levels of pSMAD2-L in resting (Rest) and immediately post-exercise (Exercise) biopsies for each subject that underwent endurance exercise are shown with values from the same subject joined by a line. e Linear regression analysis identified a significant correlation between JNK phosphorylation and pSMAD2-linker phosphorylation. *P < 0.05 vs Rest by two-way ANOVA and Tukey’s Multiple Comparison Testing. pSMAD2L, linker-region phosphorylated SMAD2; pJNK, phosphorylated (active) C-Jun N-terminal Kinase. Different exposures are shown for the P54 and P46 splice forms of JNK, as the P54 band appeared darker in human skeletal muscle
