Fig. 2 | Nature Communications

Fig. 2

From: Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1

Fig. 2

Subunit rotation and head swivel observed in RF3-70S and termination state I–IV complexes. a–e Schematic representation (upper row) and SSU structures illustrating the degree of rotation relative to non-rotated RF1-API-70S reference structure26 as shown for a RF3-70S, b state I, c state II, d state III and e state IV. The distance each atom shifts relative to the reference structure is directly coloured on the SSU (second row), shown as coloured lines connecting the same atoms between the reference and the shifted structure (third row). Superimposition of cryo-EM maps (row four) of shifted SSU (yellow) relative to reference SSU (grey) based on LSU alignments, with degrees of SSU rotation (clockwise as viewed from intersubunit interface) and head swivel indicated

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