Fig. 4 | Nature Communications

Fig. 4

From: Visualization of translation termination intermediates trapped by the Apidaecin 137 peptide during RF3-mediated recycling of RF1

Fig. 4

Small subunit rotation leads to RF3 accommodation on the large subunit. a Two views of the cryo-EM maps of the 70S ribosome (different shades of blue) and RF3 (different shades of green) from states I–IV, illustrating the coupled rotation of the SSU and RF3 relative to the LSU. b Comparison of the binding site of RF3 in state I (pale cyan, sI:RF3) and state IV (teal, sIV:RF3) relative to ribosomal protein uL6 (red) and sarcin-ricin loop (SRL, grey). The distance shifted of each domain (d1–d3) of RF3 between states I and IV is indicated. c View showing the G domain of RF3 in state I (pale cyan, sI:RF3) with switch I (teal), switch II (slate), GDPCP and proximity to SRL (grey), ribosomal proteins uL14 (tan) and bL19 (green)

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