Fig. 6
Myrf deletion from PDGFRα+ cells does not impair motor recovery following moderate thoracic contusive SCI. a Time course of locomotor function evaluated by open field BMS. While Myrf ICKO and controls did not differ after SCI (F(3, 39) = 286.0, P < 0.001; injured Myrf ICKO vs. control P = 0.518), both SCI groups were statistically different from uninjured controls at all time-points postinjury (P < 0.001). b On the BMS subscore, there is no difference between Myrf ICKO and controls (F(3, 39) = 388.0, P < 0.001; injured Myrf ICKO vs. control P = 0.966). c There is no difference between Myrf ICKO and controls in the percentage of errors (error/error + success) on the horizontal ladder after SCI (F(3, 38) = 25.86, P < 0.001, injured Myrf ICKO vs injured control Tukey’s post hoc P=0.942). d An illustration of paw recordings from the Catwalk along with parameters in g–i used to assess gait. LH left hindlimb; LF left forelimb; RF right forelimb; RH right hindlimb. e Example of the time course in which a paw is in contact with platform (colored boxes). f Example recordings of three full step cycles from the Catwalk prior to injury and tamoxifen dosing, at three WPI, and at six WPI. g–l No differences in gait were observed between Myrf ICKO and controls either with or without an injury on g hindlimb stride length (F(3, 37) = 44.13, P < 0.001; injured Myrf ICKO vs. injured control P = 0.977). h Hindlimb base of support (F(3, 37) = 48.09, P < 0.001; injured Myrf ICKO vs. injured control P = 0.630). i Combined paw position (F(3, 37) = 52.74, P < 0.001; injured Myrf ICKO vs. injured control P = 0.983). j Hindlimb duty cycle (F(3, 37) = 0.933, P = 0.435; injured Myrf ICKO vs. injured control P = 0.738). k Percent of run with one or two paws on the platform (F(3, 37) = 17.47, P < 0.001; injured Myrf ICKO vs injured control P = 0.651). l Three or four paws on the platform (F(3, 37) 15.46. P < 0.001; injured Myrf ICKO vs. injured control P = 0.934). Groups were compared at all post injury time points. All statistical comparisons were made using a two-way repeated measures ANOVA, and a Tukey’s post hoc for individual group differences. Error bars are mean ± SEM
