Fig. 5
From: Directed evolution of CRISPR-Cas9 to increase its specificity
Base editor (BE3) on-target and off-target activities measured in HEK293T cells. (−) indicates the absence of sgRNA. Substitutions were measured using targeted deep sequencing. Substitution of C5 (represented by green type) to T was measured. The PAM is shown in blue. Error bars indicate s.e.m. (n = 3)
