Fig. 1
TTR-11 is required for efficient axon regeneration. a Genomic structures of the ttr-11 and ttr-57 genes and the svh-13/F46B3.3 and F46B3.18 RNAi clone. The svh-13 RNAi knocks down both the ttr-11 and ttr-57 genes. Boxes and thick lines indicate exons and introns, respectively. The blue line indicates the region targeted by the svh-13 RNAi. The magenta bold lines underneath indicate the extent of the deleted region in each deletion mutant. b Positive and negative regeneration in D-type motor neurons. The positive and negative regeneration examples are from a wild-type animal and a ttr-11 mutant, respectively, 24 h after laser surgery in the L4 stage. In wild-type animals, a severed axon has regenerated a growth cone (arrow) and ~75% of the cut axons are scored as regenerating. In ttr-11 mutants, proximal ends of axons often failed to regenerate (arrowhead). Scale bar = 10 μm. c Regeneration of D-type motor neurons. Percentages of axons that initiated regeneration 24 h after laser surgery in the L4 stage are shown. The number (n) of axons examined are shown. Error bars indicate 95% confidence intervals (CIs). *P < 0.05, **P < 0.01 as determined by Fisher’s exact test. NS: not significant
