Fig. 4

Axon injury induces PS exposure. a Localization of MFG-E8-C2::GFP after axon injury. Fluorescent images of severed axons in animals carrying Phsp::ss::mfg-e8-c2::gfp or Phsp::ss::mfg-e8-c2(AAA)::gfp and Punc-47::mcherry are shown. D neurons are visualized by mCherry under control of the unc-47 promoter. Images were taken just before (uncut) or 1 h after laser surgery (cut). Twenty animals were examined for each condition. All 20 observations showed similar patterns. Arrowheads indicate the sites of laser surgery. Schematic diagrams of GFP localization are shown in the lower part. Scale bar = 10 μm. b The relative fluorescent intensities of MFG-E8-C2::GFP around D neurons with (+) or without (−) laser surgery. Scores were calculated from images taken just before or 1 h after laser surgery. Quantification of MFG-E8-C2::GFP is described in Methods. Twenty animals were examined for each condition. Error bars indicate SEM. ***P < 0.001 as determined by unpaired t test. c Percentages of axons that initiated regeneration 24 h after laser surgery in the L4 stage. Axotomy was performed after heat shock as described in Methods. The number (n) of axons examined are shown. Error bars indicate 95% CI. **P < 0.01 as determined by Fisher’s exact test. NS: not significant