Fig. 5
CED-7 is required for efficient axon regeneration. a Domain structures of C. elegans ANOH-1, CED-8, and CED-7. Schematic domain diagrams of C. elegans ANOH-1, CED-8, CED-7 and mammalian counterparts (TMEM16F, Xkr8, and ABCA1) are shown. Transmembrane domains and nucleotide-binding domains (NBD) are indicated by black and blue colors, respectively. b, e Percentages of axons that initiated regeneration 24 h after laser surgery in the L4 stage. The number (n) of axons examined are shown. Error bars indicate 95% CI. **P < 0.01 as determined by Fisher’s exact test. NS: not significant. c Localization of MFG-E8-C2::GFP after axon injury. Fluorescent images of severed axons in animals carrying Phsp::ss::mfg-e8-c2::gfp and Punc-47::mcherry are shown. D neurons are visualized by mCherry under control of the unc-47 promoter. Each image was taken at 1 h after laser surgery. Twenty animals were examined for each condition. All 20 observations showed similar patterns. Arrowheads indicate the sites of laser surgery. Scale bars = 10 μm. d The relative fluorescent intensities of GFP around D neurons with laser surgery. Scores were taken at 1 h after laser surgery. Quantification of MFG-E8-C2::GFP is described in Methods. Twenty animals were examined for each condition. Error bars indicate SEM. ***P < 0.001 as determined by unpaired t test
