Fig. 2

Four-week-old A129 mice elicit specific immune response against ZIKV PE243. Four-week-old A129 mice were inoculated intravenously with 2 × 10⁵ PFU of ZIKV strain PE243. Uninfected mice were used as control. a Representative dot plot and frequency of protein E ZIKV tetramer positive cells among CD8⁺ T cells or total CD8⁺CD62L⁻CD44⁺ cells (effector). Data were obtained from ZIKV PE243-infected A129 mice at days 3 (n = 4), 5 (n = 5), and 7 (n = 5) postinfection and compared to uninfected mice (n = 3). For intracellular cytokines analyzes by means of flow cytometry, splenocytes were obtained from uninfected A129 mice (n = 3–6) or ZIKV PE243-infected A129 mice (n = 4–8) at day 7 postinfection, restimulated in vitro using UV-inactivated ZIKV during 72 h in the presence of brefeldin A added in the last 8 h. b Representative dot plot and frequency of IFNγ and TNFα among CD8⁺ T cells. c Representative dot plot and frequency of IFNγ and TNFα among CD4⁺ T cells. d Serum IgM and IgG anti-ZIKV VLP or anti-ZIKV-EDIII as well as serum IgG1 and IgG2a anti-ZIKV-VLPs in ZIKV PE243-infected A129 mice (n = 8), compared to uninfected A129 mice (n = 4). Results are shown as mean. Data are representative of two independent experiments in d, data are presented as a pool of two independent experiments in b and c, experiments were performed once in a. Data were analyzed by Student’s t test. * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001; **** p ≤ 0.0001; ns not significant