Fig. 5
IFNγ, CD8+ T and B cell are crucial in CD4+ T cell protection against ZIKV MR766. Recipient AG129 mice or A129 mice depleted of B cell or CD8+ T cells were inoculated intravenously with 2 × 105 PFU of lethal ZIKV MR766. Simultaneously, the animals received immune splenocytes (5 × 107/mouse) or immune CD4+ T cells (5 × 107/mouse) obtained from ZIKV PE243-infected A129 donor mice at 7 days postinfection. Uninfected mice were used as donor of naive splenocytes to MR766-infected A129 control mice (n = 8). a Body weight gain and b lethality of recipient AG129 mice that received immune splenocytes (n = 6) or immune CD4+ T cells (n = 4) monitored for up to 7 days postinfection. c Body weight gain and d lethality of recipient A129 mice depleted of B cells (n = 5) or CD8+ T cells (n = 4) that received immune CD4+ T cells monitored for up to 15 days. IgG2a isotype control were used to exclude non-specific effects of B cell depleting antibodies (n = 8). Results are shown as mean ± standard deviation in a and c. Data are presented as a pool of two independent experiments in a–d. Survival data were analyzed by log rank test. * p ≤ 0.05; *** p ≤ 0.001
