Fig. 2

Local and systemic depletion of ILCs in untreated SIV infection. a Frequencies of MLN ILCs in healthy uninfected RMs (N = 10), untreated acute and chronic SIV-infected RMs (N = 10) (N = 11), chronic SIV⁺ RMs receiving ART (N = 6), and SIV-infected ECs (N = 4). Determined as a proportion of viable CD45⁺ hematopoietic cells. b Summary data of CD3-c-Kit⁺ cell number per area of paracortical region in the MLN. c Relationship between c-Kit⁺ LC3 proportions assessed by flow cytometry and c-Kit⁺ ILC3 enumeration by microscopy. d Frequencies of ILC3s in jejunal cell suspensions. e Correlation of NKp44⁺ ILC3 frequencies in animals with matching MLN and jejunal samples at the time of necropsy. f Frequencies of ILCs in axillary lymph nodes. g Relationship between NKp44⁺ ILC3 frequencies in the MLN and sCD14 in plasma. Statistical significance was calculated using the Mann–Whitney test. A Pearson's correlation was calculated for panels c, e, and g. ns = P > 0.05, * = P ≤ 0.05, ** = P ≤ 0.01, *** = P ≤ 0.001, and **** = P ≤ 0.0001