Fig. 5
From: HIF-2α-pVHL complex reveals broad genotype-phenotype correlations in HIF-2α-driven disease
A subset of class 1 mutations specifically disrupts PHD2-mediated hydroxylation. a, b Biotinylated HIF-2α peptides (residues 523–542) were immobilized on streptavidin- agarose beads and incubated with HIS6-PHD2 for 1 h at room temperature prior to incubation with in vitro transcribed and translated (IVTT) pVHL. Streptavidin beads were pulled down (PD) and levels of HA-tagged pVHL were visualized via immmunoblotting (IB). c HEK293a cells were co-transfected with FLAG-VHL and HA-HIF-2α constructs. FLAG was immunoprecipitated (IP) and levels of HA-tagged HIF-2α were visualized via immunoblotting (IB). * denotes uncharacterized band
