Fig. 3
From: ZCCHC3 is a co-sensor of cGAS for dsDNA recognition in innate immune response
ZCCHC3 is essential for host defense against DNA virus in mice. a Effects of ZCCHC3-deficiency on serum levels of IFN-β, CXCL10, and IL-6 induced by DNA viruses. Zcchc3+/+ and Zcchc3−/− mice (n = 6–9 per strain, 8 weeks old) were infected with HSV-1, VACV or MCMV (3 × 107, 5 × 106, and 1 × 104 PFU per mouse respectively) for 6 h before measurement of the indicated serum cytokines by ELISA. Each symbol represents an individual mouse. b Measurement of viral genomic copy numbers and viral titers. Zcchc3+/+ and Zcchc3−/− mice (n = 3 per strain, 8 weeks old) were infected with HSV-1 at 3 × 107 PFU per mouse for 6–7 days. HSV-1 genomic copy numbers and viral titers in the brains of infected mice were quantified by qPCR and plaque assays respectively. c Effects of ZCCHC3-deficiency on HSV-1-or VACV-induced death of mice. Zcchc3+/+ and Zcchc3−/− mice (n = 7 per strain for HSV-1, n = 6 per strain for VACV, 8 weeks old) were intra-nasally infected with HSV-1 at 3 × 107 or VACV at 5 × 106 PFU per mouse, and the survival rates of mice were monitored daily. d Effects of ZCCHC3-deficiency on HSV-1-induced inflammation in the lungs of mice. Sex and age-matched Zcchc3+/+ and Zcchc3−/− mice (n = 3 per strain, 8 weeks old) were intra-nasally infected with HSV-1 at 3 × 107 PFU per mouse for 6–7 days and lung sections were analyzed by H&E staining. Scale bars, 100 μm. *P < 0.05, **P < 0.01 (unpaired t test (a, b) or log-rank test (c). Data are representative of at least two experiments with similar results
