Fig. 4
From: Regulation of striatal cells and goal-directed behavior by cerebellar outputs
Dorsal striatal iMSNs receive information from all the cerebellar lobules. a Stereotaxic injection and analysis scheme. Injections in A2A-cre mice (N = 3) are initiated at P11-P12. The procedure is as in Fig. 3, except performing an additional injection of AAV2-DiO-G/AAV2-cre-GFP at P11-P12 to express viral glycoprotein in the DCN. b Example of retrogradely labeled Purkinje cells in Crus 1 (arrows) marked by mCherry expressed from the transsynaptic rabies virus. Scale bar: 200 µm. c Location of 87 retrogradely labeled Purkinje cells from tracings performed in three mice. Cell positions are mapped onto an unfolded map of the cerebellum. Scale bar: 5 mm. d Number of transsynaptically labeled Purkinje cells by cerebellar lobule. Note lobule 1/2, lobule 6 and Crus 1/Sim have the highest number of cells. Anatomical annotations: Cop copula of the pyramis; Crus 1 crus 1 of the ansiform lobule; Crus 2 crus 2 of the ansiform lobule; FI flocculus; Sim simple lobule; PFI paraflocculus; PM paramedian lobuleÂ
