Fig. 7
From: Regulation of striatal cells and goal-directed behavior by cerebellar outputs
Striatum-specific silencing of cerebello-striatal connectivity. a Stereotaxic injection scheme for local silencing experiments. For local infusion of CNO, mice are bilaterally cannulated in the dorsal striatum. b Timeline and analysis scheme of local silencing experiments. See text and methods for details. c Overview (left) and ILN enlargement (right) of dorsal striatum and thalamic nuclei in mice at the end of the experiment. Cells expressing hM4Di-mCherry fusion protein (red) are observed in parafascicular nucleus (PaF) and centromedial nucleus (CM). The spread of Alexa488 dextran applied through the cannula in the striatum is shown in green (N = 11 mice). d High magnification image of hM4Di-mCherry positive axons in the dorsal striatum. This reveals efficient transport of the hM4Di-mCherry fusion protein into the thalamo-striatal axons. e Example open field tracks of control ILNtdTom (tdTom) and ILNhM4Di (hM4Di) expressing mice (targeted with intersectional viral approach described above). Mice were locally infused with CNO (30 μM, 800 nl/side) 35 min before the test. f Quantification of distance traveled in open field, velocity, move segments, and stop segments of control ILNtdTom and ILNhM4Di mice (intersectional viral approach described above, N = 11 and 10 mice, respectively; mean ± SEM, unpaired t-test for open field, velocity and move segments. For stop segments, Mann-Whitney test, two-tailed). g T-maze forced alternation. h Mice were trained in a forced alternation paradigm with food reward. The percentage of mice reaching the training criterion after each day is plotted. ILNtdTom (tdTom) and ILNhM4Di (hM4Di) mice (intersectional viral approach described above, N = 11 and 10 mice, respectively)
