Fig. 7
From: Hapten-mediated recruitment of polyclonal antibodies to tumors engenders antitumor immunity

VEGF-αGal mediated recruitment of antibodies from human serum to tumor biopsies. Frozen section of normal and tumor biopsies were first incubated with VEGF–DNP or VEGF-αGal, then with human serum and stained with either DAPI (blue) or with mouse anti-human IgM-Alexa488 A (green) and visualized by confocal microscopy at ×40 magnification. a Renal cell cancer (RCC) and matched normal tissue. b Colon biopsy was incubated with VEGF-αGal or VEGF–DNP and three additional human sera. c Tumor biopsies from colon (shown in b), endometrium, kidney (different from a), and melanoma were incubated with VEGF–αGal or VEGF–DNP conjugate and the human serum used in a. Scale bar = 25 μm. (n = 2)