Fig. 2
From: Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals
miRNAs show directional mobility. a Diagram illustrating the expression domains of the SUC2 (yellow), ATML1 (green), ATHB8 (red), and RbcS (blue) promoters, as verified in Supplementary Fig. 2a. b Transverse section of a p35S:3xNLS-GFP (no miRGFP) hypocotyl shows ubiquitous GFP expression. c–f The patterns of GFP silencing in lines expressing miRGFP in c mesophyll (pRbcS:miRGFP), d phloem companion (pSUC2:miRGFP), e procambial (pATHB8:miRGFP), and f epidermal (pATML1:miRGFP) cells reveal directionality in miRGFP mobility, with miRGFP moving out but not into the phloem pole. Note, pATHB8:miRGFP lines show a cell autonomous pattern of GFP silencing. g Small RNA qRT-PCR shows that miRGFP levels are significantly (p < 0.001, two-sided Student’s t-test) higher in pATHB8:miRGFP vs. pATML1:miRGFP hypocotyls. Relative miRGFP levels (means ± SE; n = 3) normalised to U6 are shown. Scale bars, 50 μm. White arrowheads, phloem poles
