Fig. 4
From: Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals
miRNAs act domain-autonomously within the shoot stem cell niche. a Diagram illustrating the expression domains of the MIR166A (yellow), SCR (green), CLV3 (red), and WUS (blue) promoters, as verified in Supplementary Fig. 2. b–g Longitudinal sections through the shoot apical meristem of b a p35S:3xNLS-GFP (no miRGFP) seedling, and lines expressing miRGFP in the c abaxial epidermis of the incipient leaf (pMIR166A:miRGFP), d meristem tunica (pSCR:miRGFP), e stem cells of the central zone (pCLV3:miRGFP), and f organising centre (pWUS:miRGFP) show domain-autonomous patterns of miRGFP-directed GFP silencing. Cells in the central zone in d are marked using a pCLV3:dsRED reporter line. In contrast to f miRGFP, g free GFP expressed in the organising centre (pWUS:GFP) moves freely throughout the shoot stem cell niche. Arrowheads, third layer of the central zone. Scale bars, 10 μm
