Fig. 5
From: Gating of miRNA movement at defined cell-cell interfaces governs their impact as positional signals
Regulated miRNA mobility within the root stem cell niche. a Diagram illustrating the expression domains of the MIR166A (yellow), SCR (green), ATHB8 (red), and WOX5 (red-green) promoters, as verified in Supplementary Fig. 2c. b–g Optical longitudinal sections through the root meristem of b a p35S:3xNLS-GFP (no miRGFP) seedling, and lines expressing miRGFP in the c endodermis and quiescent centre (QC) (pSCR:miRGFP), d endodermis (pMIR166A:miRGFP), e stele, QC and columella (pATHB8:miRGFP), and f QC (pWOX5:miRGFP), show that miRGFP acts domain-autonomously within the niche but moves between determined daughter cells of the root meristem. Note: persistence of GFP fluorescence in cells of the lateral root cap is likely explained by a low density of plasmodesmatal connections to these cells37, 38. In contrast to f miRGFP, g free GFP expressed in the QC (pWOX5:GFP) moves freely throughout the root apex. Asterisks, endodermal cells; arrowheads, QC. Scale bars, 20 μm
