Fig. 5
From: PARP2 mediates branched poly ADP-ribosylation in response to DNA damage
APLF recognizes the branched PAR. a Diagrammatic representation of the sample preparation. PAR was incubated with APLF PBZ or the WWE domain of RNF146, and then partially digested by PARG. The protein-associated residues were further treated with PPase and AP, and then subjected to LC–MS/MS. APLF PBZ (b) or the WWE domain of RNF146 (c) bound residues were examined. d The recruitment of APLF to DNA lesions is dependent on PARP2. GFP-APLF was expressed in U2OS or PARP2-null cells. The recruitment kinetics of APLF was examined following DNA damage at various time intervals. Scale bar represents 5 μm. Data are represented as mean ± s.d. as indicated from three independent experiments. Significance of differences was evaluated by Student’s t-test. NS: non significant; ***statistically significant (p < 0.001)
