Table 1 Kinetic characterization of the three 5-deoxyribose-metabolizing enzymes
From: Salvage of the 5-deoxyribose byproduct of radical SAM enzymes
Enzyme | Substrates | Co-substrates | Km (µM) | kcat (s−1) | kcat/Km (M−1 s−1) |
|---|---|---|---|---|---|
DrdK (kinase) | 5-Deoxyribose | ATP | 178 ± 6 | 15.4 ± 0.4 | 8.7 ± 0.4 × 104 |
5-Methylthioribose | ATP | 72 ± 20 | 6.2 ± 0.4 | 8.5 ± 2.4 × 104 | |
ATP | 5-Deoxyribose | 216 ± 63 | 17.1 ± 2.5 | 7.9 ± 2.6 × 104 | |
ATP (ATPase activity) | – | 84 ± 21 | 3.0 ± 0.3 | 3.6 ± 1.0 × 104 | |
DrdI (isomerase) | 5-Deoxyribose 1-P | – | 22 ± 7 × 103 | 3.5 ± 0.7 | 1.6 ± 0.6 × 102 |
DrdA (aldolase) | 5-Deoxyribulose 1-P | – | 36 ± 3 | 6.3 ± 0.2 | 1.7 ± 0.1 × 105 |
