Fig. 2
From: ATP activates bestrophin ion channels through direct interaction
The influence of ATP on hBest1-mediated Ca2+-dependent Cl− current in human RPE cells. a Confocal image showing plasma membrane localization of hBest1 in human RPE (Scale bar, 10 μm). b Representative current traces recorded from a WT iPSC-RPE in the absence (left) or presence (right) of 2 mM ATP (Scale bar, 1 nA, 125 ms). c ATP-dependent activation of surface currents in WT iPSC-RPE. Steady-state current density recorded at +100 mV plotted vs. free [ATP]i, n = 5–6 for each point. The plot was fitted to the Hill equation. d Bar chart showing the steady-state current amplitudes in the presence of ATP (10 mM) without Ca2+, Ca2+ (0.6 μM) without ATP, Ca2+ (0.6 μM) + ATP (10 mM), Ca2+ (0.6 μM) + ATPγS (10 mM), and Ca2+ (0.6 μM) + ATP (10 mM) + NFA (100 μM) in WT iPSC-RPE, n = 5–6 for each bar. *#P < 0.05 compared to currents with Ca2+ and Ca2+ + ATP, respectively, using one-way ANOVA and Bonferroni post hoc analyses. All error bars in this figure represent s.e.m.
