Fig. 3 | Nature Communications

Fig. 3

From: Palladium prompted on-demand cysteine chemistry for the synthesis of challenging and uniquely modified proteins

Fig. 3

Schematic representation of palladium-mediated modification of peptide 3 at selected Cys sites. a Reaction at time zero: main peak corresponds to peptide 3 with the observed mass 1473.0 Da ± 0.4 Da, calcd 1473.7 Da (average isotopes). b 1st alkylation reaction, main peak corresponds to mono-alkylated peptide 3 with the observed mass 1616.0 Da ± 0.5 Da, calcd 1616.7 Da (average isotopes). c Thz opening: main peak corresponds to Thz-deprotected peptide 3 with the observed mass 1604.0 Da ± 0.7 Da, calcd 1604.7 Da (average isotopes). d 2nd alkylation reaction: main peak corresponds to di-alkylated peptide 3 with the observed mass 1747.0 Da ± 0.4 Da, calcd 1747.7 Da (average isotopes). e Acm deprotection: main peak corresponds to Acm-deprotected peptide 3 with the observed mass 1676.0 Da ± 0.4 Da, calcd 1676.7 Da (average isotopes). f 3rd alkylation reaction: main peak corresponds to tri-alkylated peptide 3 with the observed mass 1733.0 Da ± 0.4 Da, calcd 1733.6 Da (average isotopes). Red sphere: acetylglycine-ethyl ester, green sphere: acetylalanine-methyl ester, purple sphere: acetamide

Back to article page