Fig. 5 | Nature Communications

Fig. 5

From: Palladium prompted on-demand cysteine chemistry for the synthesis of challenging and uniquely modified proteins

Fig. 5

Total chemical synthesis of H2AK119DHAUb. a The H2A sequence where the two-ligation sites are underlined. b Synthesis of H2AK119DHAUb and analytical HPLC and mass traces for purified H2AK119DHAUb with the observed mass 22495 ± 2.7 Da, calcd 22498 Da (average isotopes), H2A in green, ubiquitin in red. N-MeNbz: N-acyl-N-methylacylurea, Nbz N-acyl-benzimidazolinone, R2 3-Mercaptopropionate, VA044 2,2’-Azobis[2-(2-imidazolin-2-yl)propane] Dihydrochloride

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