Fig. 6 | Nature Communications

Fig. 6

From: Palladium prompted on-demand cysteine chemistry for the synthesis of challenging and uniquely modified proteins

Fig. 6

NCP(H2A-UbDHA) labeling with Calypso/ASX DUB. a Schematic of nucleosome probe generation and labeling with Calypso/ASX DUB. H2A in green, ubiquitin in light red, H4 in orange, H3 in blue, H2B in red and DNA in gray b EMSA analysis suggests the formation of a stable product, even in serial dilutions c SDS-PAGE analysis of the labeling reaction between NCP(H2A-UbDHA) and Calypso/ASX DUB reveals the generation of a band with higher molecular weight, corresponding to a Calypso/ASX-H2A-UbDHA crosslinked species. d Western blot analysis of samples shown in (c) using ubiquitin antibody shows high-molecular-weight species, which contains ubiquitin formed upon reaction of Calypso/ASX with NCP(H2A-UbDHA)

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