Fig. 2

Dependence of water freezing efficiency on the volume and viscosity of sealing agents. a Effect of sealing oil (MO) volume on ff post 1-day DSC at different temperatures. n = 6, N = 70. b Side view of corresponding samples of a. MO includes Oil Red O for staining and imaging. 0 oil, 0.01 ml, 0.1 ml, 0.15 ml, 0.5 ml, and 3.5 ml indicate no seal, ring seal, partial seal, critical seal (just complete surface seal), standard seal, and over seal by MO, respectively. c Effect of viscosity of sealing agents on ff post 1-day DSC at −16 °C. The sealing agents are hydroxy (OH) terminated polydimethylsiloxane (PDMS) of different chain lengths and viscosities. n = 5, N = 56. Error bars represent standard deviations