Fig. 5 | Nature Communications

Fig. 5

From: The dual methyltransferase METTL13 targets N terminus and Lys55 of eEF1A and modulates codon-specific translation rates

Fig. 5

MT13-N catalyzes methylation of eEF1A-Lys55. a Volcano plot showing differences in the mean MS intensities for lysine methylation sites in HAP-1 WT and METTL13 KO cells. Curved lines represent the significance cutoff (FDR = 0.01 and s0 = 0.1). The significant sites, dimethylation of Lys55 in eEF1A (eEF1A-K55-Me2), and monomethylation of Lys1163 in APOB (APOB-K1163-Me1), are indicated. b Ion chromatograms representing the different methylated forms of eEF1A-Lys55 in WT, KO, and KO cells complemented with FLAG-tagged METTL13 (KO + METTL13-FLAG). c Evaluation of a Lys55-to-Arg (K55R) mutant of eEF1A1 as a substrate for MT13-N. eEF1A1 constructs were incubated with MT13-N as indicated and methylation was visualized by fluorography (top panel). The corresponding Ponceau S-stained membrane is shown to assess for protein loading (bottom panel)

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