Fig. 1
From: Reviving the Weizmann process for commercial n-butanol production
Metabolic engineering of Clostridium acetobutylicum for n-butanol production. a Elimination of by-products formation (strain CAB1057) by gene deletion. ldhA lactate dehydrogenase, ctfAB acetoacetyl-CoA-acetate CoA-transferase, adc acetoacetate decarboxylase, ptb phosphotransbutyrylase, buk butyrate kinase, adhE2 bifunctional NAD+-dependent aldehyde and alcohol dehydrogenase. b Optimization of acetyl-CoA conversion to n-butanol (Strain CAB1060) using a chimeric pathway derived from three different microorganisms. Blue C. acetobutylicum; black E. coli, red C. kluyveri; Green boxes are Rex binding sites. atoB acetoacetyl-CoA thiolase/synthase, hbd1 NADP+-dependent 3-hydroxybutyryl-CoA dehydrogenase, crt crotonase, bcd-etfAB butyryl-CoA dehydrogenase
