Fig. 2 | Nature Communications

Fig. 2

From: Variation in bradyrhizobial NopP effector determines symbiotic incompatibility with Rj2-soybeans via effector-triggered immunity

Fig. 2

Characterization of spontaneous mutants overcoming Rj2-mediated incompatibility. a Number of root nodules and nitrogen fixation activity of the roots of G. max cv. Hardee at 28 days after inoculation with USDA 122 and its spontaneous mutants. The data of nodule number shown are representative of two independent experiments. Nitrogen fixation activity was measured by acetylene reduction assay. Error bars show SEM (n = 3). Significant differences from USDA 122 by Dunnett’s multiple comparison test: *P < 0.05, **P < 0.01. b Shoots and roots of G. max cv. Hardee (Rj2) at 28 days after inoculation with USDA 122 or its spontaneous mutants. Scale bars, 5 cm (for shoots) or 0.5 cm (for roots). c Positions of mutations in the genomes of spontaneous mutants. Bold horizontal lines indicate the symbiosis island (SI, 672 kb). ISRj1 and ISRj2, insertion sequence elements

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