Fig. 4
Effect of nopP swapping between USDA 122 and 110T on symbiosis with Hardee. a Western blotting analysis of NopP secreted into culture supernatants from USDA 110, 110nopP122, and nopP deletion mutant of USDA 110T (110∆nopP). Strains were grown in the presence of 10 μM genistein. The position of NopP is indicated by arrow. b Differences in amino acid residues in NopP between B. diazoefficiens USDA 122 and 110T. c Shoots and roots of cv. Hardee at 28 days after inoculation with USDA 122, 110T, or strains with swapped nopP (122nopP110, 110nopP122). Scale bars, 5 cm (for shoots) or 1 cm (for roots). d Number of nodules formed on roots at 28 days after inoculation. The data shown are representative of three independent experiments. Error bars show SEM (n = 4). e Number of infection threads (ITs). ITs were counted on whole region of main roots at 2 days after inoculation. Error bars show SEM (n = 3). *P < 0.05, **P < 0.01 by two-tailed Student’s t-test
