Fig. 1

T cell-specific deletion of Senp3 perturbs T cell homeostasis. a Flow cytometric analysis of the frequency of naive (CD44^loCD62L^hi) and memory-like (CD44^hiCD62L^lo for CD4⁺ and CD44^hi for CD8⁺ T cells) CD4⁺ and CD8⁺ T cells in total splenocytes from 8-week-old Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice. b Flow cytometric analysis of the percentage of IFN-γ-producing and IL-17-producing CD4⁺ and CD8⁺ T cells in the spleen of 8-week-old Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice. c, d Flow cytometric analysis of the frequency of naive and memory-like (c) or IFN-γ-producing and IL-17-producing (d) T cells in total splenocytes from 8-month-old Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice. e Hematoxylin-eosin staining of the indicated tissue sections from 8-month-old Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice, showing immune cell infiltrations into the Senp3^fl/flCd4-Cre tissues (arrows). Bars, 100 μm. f, g Quantification of CD4⁺ and CD8⁺ T cells (f) and percentage of CD4⁺ and CD8⁺ T cells expressing CD69 (g) in the lungs and livers of 8-month-old Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice. h Survival curves of Senp3^+/+Cd4-Cre and Senp3^fl/flCd4-Cre mice (n = 10). Data are representative of three or more independent experiments. Error bars are the mean ± SEM values. n = 5 or 10. Two-tailed unpaired Student’s t tests were performed. *P < 0.05; **P < 0.01