Fig. 2

Loss-of-function analysis identifies critical components of the RAS interactomes. Waterfall plot of the sgRNA log₂ enrichment score of each of the 474 genes encoding interactome proteins targeted by Cas9/CRISPR-mediated loss-of-function (filled circle) in cells transformed by KRAS^G12V (top), NRAS^G12V (middle), or HRAS^G12V (bottom). Enrichment scores for control ribosomal and BRAF genes, as well as scramble sgRNA, are shown for reference. Top 1% of positively and negatively enriched genes are labeled (black: enriched in cells transformed by multiple RAS isoforms; red: enriched in cells transformed by only one RAS isoform)