Table 1 Acylation of a series of peptides with GDL

From: Selective N-terminal acylation of peptides and proteins with a Gly-His tag sequence

Peptide

Peptide sequence

Acylation (%)a

2

LRFKFY-NH2

3.0 ( ± 0.7)

3

GLRFKFY-NH2

17.3 ( ± 0.7)

4

GSLRFKFY-NH2

15.7 ( ± 1.6)

5

HLRFKFY-NH2

29.8 ( ± 0.7)

6

HHHLRFKFY-NH2

64.4 ( ± 13.9)

7

GHLRFKFY-NH2

45.2 ( ± 1.7)

8

GSHLRFKFY-NH2

33.4 ( ± 1.9)

9

GHHLRFKFY-NH2

63.7 ( ± 8.6)

10

GHHHLRFKFY-NH2

81.9 ( ± 3.5)

11

AHHHLRFKFY-NH2

27.4 ( ± 4.3)

12

VHHHLRFKFY-NH2

4.3 ( ± 7.5)

  1. aConversion of peptides 2–12 (1 mM) after treatment with 25 equiv. of GDL (1) in 200 mM HEPES buffer at pH 7.5 at room temperature for 2 h, as determined by LC–MS (UV, 215 nm). Standard deviations are based on triplicate measurements. The His acylation tags and compound numbers are indicated in bold
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