Fig. 3

A lack of MKRN1 expression reduces obesity in mice placed on an HFD. Male WT and MK1^−/− mice fed a chow diet or an HFD for 16 weeks. a Representative images of male WT and MK1^−/− mice fed a chow diet (left) or an HFD (right). b The body weights of male mice on a chow diet (left) or on an HFD (right) were measured every 4 days. c Body weight gain in male mice fed a chow diet or an HFD for 12 weeks (chow, WT n = 10 and MK1^−/− n = 12; HFD, WT n = 16 and MK1^−/− n = 18). d, e Fat volume (Vis visceral, SQ subcutaneous, Abd abdominal adipose tissue) and lean mass weight were calculated (e) through micro-CT imaging (d) of WT and MK1^−/− mice on an HFD (n = 5 mice per group). P-value compared with WT. f, g Representative images of epididymal fat (left), subcutaneous fat (right) (top, scale bar = 1 cm) and H&E staining (bottom, scale bar = 50 (Epi) or 100 (Sub) µm) (f) and quantitative analysis of the adipocyte area (g) in male WT and MK1^−/− mice fed an HFD (n = 5 mice per group). P-value compared with WT. h Plasma concentrations of TG and cholesterol in 24 h-fasted male mice fed an HFD (n = 6 mice per group). The images in a, d and f are representative images from the respective experiments. Two-tailed Student’s t-test; ^*P ≤ 0.05, ^**P ≤ 0.01, ^***P ≤ 0.001, n.s. not significant. Mean ± s.d.