Fig. 6

Ablation of hepatic MKRN1 improves hepatic steatosis in diet-induced obese mice. a Male B6 mice (6 weeks old) were fed an HFD for 9 weeks and then injected with either PBS or adenoviruses expressing GFP via the tail vein (Ad_control, Ad_shMK1 #1 and #2 (shRNA targeting MKRN1)). After 1 week of continuous HFD feeding, the mice were sacrificed and analysed. b Immunoblot analysis of GFP expression in extracts from the indicated tissues (Sub subcutaneous fat, Epi epididymal fat) of mice infected with adenovirus (n = 2). c, d Liver (e) and BAT (d) lysates from adenovirus-injected mice were analysed by immunoblotting as indicated. e Body weights of male mice fed with an HFD were measured in every 2 days for 8 days (n = 5 mice per group). f Representative H&E staining of liver sections. Scale bar = 50, 100 and 500 µm. g Liver TG levels were measured. h Lipogenic enzymes were analysed via quantitative real-time PCR (n = 5 mice per group). i Representative H&E staining of epididymal fat (top) and subcutaneous fat (bottom) sections. Scale bar = 500 µm. j The weights of fat tissues were recorded following sacrifice. k Liver TG levels (n = 5 mice per group). Plasma lipid (TG, cholesterol and FFA) concentrations in 24-h-fasted mice (n = 5 mice per group). The data are presented as the mean ± s.d. Two-tailed Student’s t-test; ^*P ≤ 0.05, ^**P ≤ 0.01, ***P ≤ 0.001.